植物血球凝集素/Lectin/PHA-M/PHA-P,~80%
- 产品编号：L0114
- 中文名称：植物血球凝集素/Lectin/PHA-M/PHA-P
- 英文名称：Lectin from Phaseolus vulgaris
- 英文别名:Phaseolus vulgaris agglutinin, PHA
- CAS号:9008-97-3
- 纯度:~80%
- EC号:232-718-7
订货信息：
产品规格价格(RMB)
- L0114-25MG(PHA-M)220
- L0114-5MG(PHA-P)110
- L0114-25MG(PHA-P)220
- L0114-100MG(PHA-P)700
性能描述
| form | lyophilized powder |
| potency | <40 μg per mL agglutination activity |
| composition | Protein, 50-70% biuret |
| impurities | salt, free |
| 保存温度 | 2-8°C |
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
PHA由腰豆（haseolus vulgaris)等植物种子提取而得的一种能凝集红细胞，白细胞的糖蛋白。本品为白色粉末，溶于生理盐水，不溶于有机溶剂。分子量128000. 本品为非特异性免疫功能刺激剂。它能促进淋巴细胞转化为淋巴母细胞；促进单核细胞转变为巨噬细胞，提高巨噬细胞吞噬功能，诱导干扰素，移动抑制因子及淋巴毒素产生；凝集癌细胞，其作用较之正常细胞约强20倍；刺激骨髓造血机能等
